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Reference type: Journal
Authors: He LM, Su YJ, Zheng YQ, Huang XH, Wu L, Liu YH, Zeng ZL, Chen ZL
Article Title: Novel cyromazine imprinted polymer applied to the solid-phase extraction of melamine from feed and milk samples.
Publication date: 2009
Journal: Journal of Chromatography A
Volume: 1216
Issue: (34)
Page numbers: 6196-6203.
DOI: 10.1016/j.chroma.2009.06.081
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4WP47NK-1/2/b66606d88b4e5085ff12b358f1af4f9d

Abstract: A water compatible molecularly imprinted polymer (MIP) using cyromazine as a mimic template, methacrylic acid as the functional polymer and ethylene glycol dimethacrylate as the cross-linker was synthesized and used to extract melamine from feed and milk samples via a molecularly imprinted solid-phase extraction (MISPE) protocol. Optimum retention of melamine on the MISPE cartridge was achieved using methanol, and the interferences in the samples were effectively washed out. The binding capacity of the polymer toward melamine was found to be about 500 μg of melamine/g of polymer. The recoveries of 2 μg and 20 μg melamine standard spiked into water extract of blank feeds and milk samples were between 83.4% and 103%, with relative standard deviation <5.6%. The cyromazine-MIP demonstrated high cross-reactivity for melamine and low affinity to cyanuric acid. The ionic bond interaction was regarded as the main factors that dominated the retention of the melamine on the MISPE cartridge
Template and target information: cyromazine, melamine
Author keywords: molecular imprinting, Solid-phase extraction, Melamine, Feed, milk

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