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Reference type: Journal
Authors: Yu JH, Zhang CC, Dai P, Ge SG
Article Title: Highly selective molecular recognition and high throughput detection of melamine based on molecularly imprinted sol-gel film.
Publication date: 2009
Journal: Analytica Chimica Acta
Volume: 651
Issue: (2)
Page numbers: 209-214.
DOI: 10.1016/j.aca.2009.08.018
Alternative URL: http://www.sciencedirect.com/science/article/B6TF4-4X24VR3-5/2/e8217e51e9010d802093ede9ee6e8089

Abstract: Multimode reader has been generally applied in immunoassay, and in the proposed paper, the 96 well micro-plate was modified with molecularly imprinted melamine sol-gel film, based on which the highly selective and high throughput detection of melamine was achieved. Melamine was imprinted into silica sol-gel films directly using phenyltrimethoxysilane and methyltrimethoxysilane as functionalized organosilicon precursors. The binding characteristic of the imprinted film to melamine was evaluated by equilibrium binding experiments and the morphology was studied by scanning electronic microscope (SEM). Scatchard analysis was carried out to estimate the binding parameters of the imprinted film. The proposed method exhibited excellent selectivity because of specific recognition of MM by molecularly imprinted film. Under the optimum conditions, the chemiluminescence (CL) intensity had a linear relationship against the concentration of melamine over the range of 0.1-50 μg mL-1 with a lower detection limit of 0.02 μg mL-1
Template and target information: melamine, MM
Author keywords: Molecularly imprinted film, 96 Well micro-plate, Melamine, chemiluminescence


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