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Reference type: Journal
Authors: Yan JY, Yan YJ, Yang JK, Xu L, Liu Y
Article Title: Combined strategy for preparation of a bioimprinted Geotrichum sp. lipase biocatalyst effective in non-aqueous media.
Publication date: 2009
Journal: Process Biochemistry
Volume: 44
Issue: (10)
Page numbers: 1128-1132.
DOI: 10.1016/j.procbio.2009.06.008
Alternative URL: http://www.sciencedirect.com/science/article/B6THB-4WM0JCB-1/2/2103e6f4c131a17ba3ea39ab47a199ac

Abstract: Geotrichum sp. lipase with enhanced activity and operational stability was prepared for use in non-aqueous media. A combined strategy comprising bioimprinting with dual imprint molecules and a co-solvent coupled to pH tuning, KCl salt activation, lecithin coating and immobilization on macroporous resin effectively enhanced the activity and operational stability of Geotrichum sp. lipase. The modified lipase exhibited 18.4-fold enhanced esterification activity towards methyl oleate synthesis, and retained 90% activity following repeated use in 10 cycles. The combined strategy exhibited a significant synergistic effect and was suitable for lipase modification, dramatically enhancing the enzyme activity and operational stability. This approach is applicable to the preparation of other enzyme biocatalysts, since the methods are effective for upgrading crude enzyme to a refined product with high activity and stability for use in non-aqueous media
Template and target information: bioimprinting
Author keywords: bioimprinting, pH tuning, Salt activation, coating, immobilization, lipase

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