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Reference type: Journal
Authors: Seidler K, Polreichová M, Lieberzeit PA, Dickert FL
Article Title: Biomimetic Yeast Cell Typing-Application of QCMs.
Publication date: 2009
Journal: Sensors
Volume: 9
Issue: (10)
Page numbers: 8146-8157.
DOI: 10.3390/s91008146

Abstract: Artificial antibodies represent a key factor in the generation of sensing systems for the selective detection of bioanalytes of variable sizes. With biomimetic surfaces, the important model organism Saccharomyces cerevisiae and several of its growth stages may be detected. Quartz crystal microbalances (QCM) with 10 MHz fundamental frequency and coated with polymers imprinted with synchronized yeast cells are presented, which are able to detect duplex cells with high selectivity. Furthermore, a multichannel quartz crystal microbalance (MQCM) was designed and optimized for the measurement in liquids. This one-chip system based on four-electrode geometry allows the simultaneous detection of four analytes and, thus, provides a monitoring system for biotechnology and process control. For further standardization of the method, synthetic stamps containing plastic yeast cells in different growth stages were produced and utilized for imprinting. Mass-sensitive measurements with such MIPs resulted in the same sensor characteristics as obtained for those imprinted with native yeast cells
Author keywords: molecular imprinting, Yeast, QCM, Bioanalyte Sensing, Process control


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