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Reference type: Journal
Authors: Ansell RJ, Ramstr�m O, Mosbach K
Article Title: Towards artificial antibodies prepared by molecular imprinting.
Publication date: 1996
Journal: Clinical Chemistry
Volume: 42
Issue: (9)
Page numbers: 1506-1512.
Alternative URL: https://www.researchgate.net/publication/14414743_Artificial_antibodies_prepared_by_molecular_imprinting

Abstract: The new technique of molecular imprinting has increasingly been adopted by research laboratories worldwide during the last few years. We have studied the use of such imprints against drugs as artificial antibody-binding mimics in competitive radioimmuno-style binding assays. The recognition sites ''molded'' in the polymers mimic the binding sites of natural antibodies in their interactions with the target antigen. Binding constants are as low as 4.0 nmol/L for a small number of well-defined sites, and cross-reactivities are similar to or better than those observed with biological antibodies. In some cases, the polymers hare been used to determine drug concentrations in human serum specimens
Author keywords: immunoassays, polymers, antibody-antigen interaction, drug assays, resolving racemic mixtures

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