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Reference type: Journal
Authors: Kamel AH, Sayour HEM
Article Title: Flow-Through Assay of Quinine Using Solid Contact Potentiometric Sensors Based on Molecularly Imprinted Polymers.
Publication date: 2009
Journal: Electroanalysis
Volume: 21
Issue: (24)
Page numbers: 2701-2708.
DOI: 10.1002/elan.200904699

Abstract: Miniaturized potentiometric membrane sensors for quinine incorporated with molecular imprinted polymer (MIP) were synthesized and implemented. Planar PVC based polymeric membrane sensors containing quinine-methacrylic and/or acrylic acid-ethylene glycol methacrylate were dispensed into anisotropically etched wells on polyimide wafers. The determination of quinine was carried out in acidic solution at pH 6, where positively charged species predominated prevalently. The suggested miniaturized planner sensors exhibited marked selectivity, sensitivity, long-term stability and reproducibility. At their optimum conditions, the sensors displayed wide concentration ranges of 4.0×10-6-1.0×10-2mol L-1 and 1.0×10-5-1.0×10-2 mol L-1 with slopes of about 61.3-55.7 mV decade-1; respectively. Sensors exhibit detection limits of 1.2×10-6 and 8.2×10-6 mol L-1 upon the use of methacrylic and acrylic acid monomers in the imprinted polymer, respectively. Validation of the assay method according to the quality assurance standards (range, within-day repeatability, between-day variability, standard deviation, accuracy, and good performance characteristics) which could assure good reliable novel sensors for quinine estimation was justified. Application of the proposed flow-through assay method for routine determination of quinine in soft drinks was assayed and the results compared favorably with data obtained by the standard fluorimetric method
Template and target information: quinine
Author keywords: molecular imprinted polymers (MIPs), Miniaturized potentiometric sensors, Flow injection analysis (FIA), Soft drinks, Urine analysis, sensors, imprinting


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