Abstract: A molecularly imprinted polymer (MIP) was prepared against histamine using zinc(II)-protoporphyrin (ZnPP) as the fluorescent functional monomer. Fluorescence of the MIP is quenched in the presence of histamine, whereas such effect is much less with the non-imprinted polymer (NMIP) or the MIP in the presence of a structurally related molecule, imidazole. The association constant for the binding of histamine to the MIP was determined to be 4500M-1 and 269M-í separately in two different concentration ranges of histamine. Determination of unbound histamine by UV-VIS absorption method showed the same results as fluorescence detection of the MIP suspended solution, suggesting that the fluorescence quenching of the MIP is due to the binding of histamine to the recognition site of ZnPP-based MIP.
Template and target information: histamine