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Reference type: Journal
Authors: Okutucu B, Çelem EB, Önal S
Article Title: Immobilization of α-galactosidase on galactose-containing polymeric beads.
Publication date: 2010
Journal: Enzyme and Microbial Technology
Volume: 46
Issue: (3-4)
Page numbers: 200-205.
DOI: 10.1016/j.enzmictec.2009.12.005
Alternative URL: http://www.sciencedirect.com/science/article/B6TG1-4Y0KWHP-1/2/d92aed2dd700ff99ae52367df191b502

Abstract: Industrial application of α-galactosidase requires efficient methods to immobilize the enzyme, yielding a biocatalyst with high activity and stability compared to free enzyme. An α-galactosidase from tomato fruit was immobilized on galactose-containing polymeric beads. The immobilized enzyme exhibited an activity of 0.62 U/g of support and activity yield of 46%. The optimum pH and temperature for the activity of both free and immobilized enzymes were found as pH 4.0 and 37 °C, respectively. Immobilized α-galactosidase was more stable than free enzyme in the range of pH 4.0-6.0 and more than 85% of the initial activity was recovered. The decrease in reaction rate of the immobilized enzyme at temperatures above 37 °C was much slower than that of the free counterpart. The immobilized enzyme shows 53% activity at 60 °C while free enzyme decreases 33% at the same temperature. The immobilized enzyme retained 50% of its initial activity after 17 cycles of reuse at 37 °C. Under same storage conditions, the free enzyme lost about 71% of its initial activity over a period of 7 months, whereas the immobilized enzyme lost about only 47% of its initial activity over the same period. Operational stability of the immobilized enzyme was also studied and the operational half-life (t1/2 was determined as 6.72 h for p-nitrophenyl α-d-galactopyranoside (PNPG) as substrate. The kinetic parameters were determined by using PNPG as substrate. The Km and Vmax values were measured as 1.07 mM and 0.01 U/mg for free enzyme and 0.89 mM and 0.1 U/mg for immobilized enzyme, respectively. The synthesis of the galactose-containing polymeric beads and the enzyme immobilization procedure are very simple and also easy to carry out
Template and target information: galactose
Author keywords: α-Galactosidase, molecular imprinting, Galactose-containing polymeric beads, Bioaffinity, immobilization


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