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Reference type: Journal
Authors: Zhang MS, Huang JR, Yu P, Chen X
Article Title: Preparation and characteristics of protein molecularly imprinted membranes on the surface of multiwalled carbon nanotubes.
Publication date: 2010
Journal: Talanta
Volume: 81
Issue: (1-2)
Page numbers: 162-166.
DOI: 10.1016/j.talanta.2009.11.052
Alternative URL: http://dspace.xmu.edu.cn/dspace/bitstream/2288/1785/1/Preparation%20and%20characteristics%20of%20protein%20molecularly%20imprinted%20membranes%20on%20the%20surface%20of%20multiwalled%20carbon%20nanotubes.pdf

Abstract: A novel protein molecularly imprinted membrane (PMIM) was synthesized on the surface of multiwalled carbon nanotubes (MWNTs) through a surface molecular imprinting technique by using bovine serum albumin (BSA) as the template molecule, acrylamide (AAm) as the functional monomer, N,N'-methylenebisacrylamide (NNMBA) as the cross-linker and ammonium persulphate [(NH4)2S2O8] as the initiator. The amounts of raw materials were optimized in this paper and the suitable amount is 0.1 g of CNTs, 0.02 g of BSA, 0.24 g of AAm, 0.1 g of MBA and 0.025 mg of (NH4)2S2O8. The selective recognition ability of PMIM/MWNTs was evaluated using adsorption experiments. Maximum adsorption capacity was 5.53 μg/mg PMIM/MWNTs and a saturation value was achieved at a BSA concentration of 0.2 mg/mL. The selectivity adsorption experiments showed that the PMIM/MWNTs also had higher adsorption capacities for BSA than for such molecules, as HSA, HB, pepsin and HRP. The PMIM/MWNTs displayed a 2.6-fold increase in affinity to BSA compared to the nPMIM/MWNTs. The PMIM/MWNTs, on the other hand, did not exhibit any significant change in affinity to other molecules compared to the nPMIM/MWNTs. In the experiment, the characteristics of the PMIM/MWNTs were analyzed using infrared spectroscopy and their configurations were observed using scanning electron microscopy
Template and target information: protein, bovine serum albumin, BSA
Author keywords: molecular imprinting technology, Multiwalled carbon nanotubes, Bovine serum albumin, protein


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