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Reference type: Journal
Authors: Tang PP, Luo ZF, Cai JB, Su QD
Article Title: A novel indirect inhibitive immunoassay for sulfamethoxazole.
Publication date: 2010
Journal: Chinese Chemical Letters
Volume: 21
Issue: (8)
Page numbers: 955-958.
DOI: 10.1016/j.cclet.2010.03.029
Alternative URL: http://www.sciencedirect.com/science/article/B8G3X-506HPRS-J/2/3da7fa60e740c559328a7ab77b208ce0

Abstract: A new indirect inhibitive immunoassay using surface plasmon resonance (SPR) coupled with molecularly imprinted polymer (MIP) was developed. A sulfamethoxazole (SMX) MIP coating capillary was produced and used as an in-tube solid phase extraction (SPE) device. The MIP coating formed a nanometer film on the inner wall of the capillary. The anti-SMX mono-antibody was inhibited by SMX extracted by the MIP coating in a dose-dependent manner. The calibration curve was generated by linear fit over the range of 0.04-10.00 ng/mL. The limit of detection was 0.01 ng/mL. This method has high sensitivity and can be performed automatically
Template and target information: sulfamethoxazole, SMX
Author keywords: sulfamethoxazole, molecularly imprinted polymer, surface plasmon resonance, Indirect inhibitive immunoassay


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