MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Lai JP, Lu XY, Lu CY, Ju HF, He XW
Article Title: Preparation and evaluation of molecularly imprinted polymeric microspheres by aqueous suspension polymerization for use as a high-performance liquid chromatography stationary phase.
Publication date: 2001
Journal: Analytica Chimica Acta
Volume: 442
Issue: (1)
Page numbers: 105-111.
DOI: 10.1016/S0003-2670(01)01115-1
Alternative URL:

Abstract: Molecularly imprinted polymeric microspheres (MIPMs) were prepared using 4-aminopyridine as imprinting molecule by aqueous suspension polymerization. The MIPMs were packed into stainless steel column (250 mm. x 4.6 mm i.d.) for selective separation and quantitative determination of 4-aminopyridine and 2-aminopyridine. The influence of mobile phases adjusted with different pH buffers (acetate solution) on capacity factors were investigated. The results showed that the capacity factor (k ') for 4-aminopyridine increased with increasing pH of mobile phase while that of 2-aminopyridine was subtly changed. The studies showed that the capacity factors for substrates were affected by both of MIPM stationary phase and mobile phase. In low pH (pH less than or equal to 5.5) mobile phase, the 4-aminopyridine was eluted prior to 2-aminopyridine and its capacity factor (k ') was lower. In high pH (pH > 5.5) mobile phase, however, the capacity factor (k ') for template molecule exhibited far higher than that of non-imprinting molecule. Therefore, the imprinted microspheres against 4- aminopyridine were used to separate and simultaneous determinate 4-aminopyridine and 2-aminopyridine. The responses of peak areas versus concentrations of substrates present good linearity with R > 0.999. This method may be used to separate and simultaneous determinate multicomponental homologous compounds or isomers, which are very expensive or unavailable or whose physicochemical properties are very similar, by changing the pH of mobile phases. (C) 2001 Elsevier Science B.V. All rights reserved
Template and target information: 4-aminopyridine

  mipdatabase.com logo imprinters do it in bulk shirt  Science Teacher peptide mug  Perpetual student shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner