Authors: Ikawa T
, Hoshino F
, Watanabe O
, Li YL
, Pincus P
, Safinya CR
Molecular Scale Imaging of F-Actin Assemblies Immobilized on a Photopolymer Surface.
Publication date: 2007
Journal: Physical Review Letters
Art. No. 018101.
Abstract: A photo-immobilization based process is presented for direct imaging of hierarchical assemblies of biopolymers using atomic force microscopy (AFM). The technique was used to investigate the phase behavior of F-actin aggregates as a function of concentration of the divalent cation Mg2+. The data provided direct experimental evidence of a coil-on-coil (braided) structure of F-actin bundles formed at high Mg2+ concentrations. At intermediate Mg2+ concentrations, the data showed the first images of the two-dimensional nematic rafts discovered by recent x-ray studies and theoretical treatments. Copyright (C) 2010 The American Physical Society
Template and target information: protein, F-actin
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