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Reference type: Journal
Authors: Kaulpiboon J, Pongsawasdi P, Zimmermann W
Article Title: Altered product specificity of a cyclodextrin glycosyltransferase by molecular imprinting with cyclomaltododecaose.
Publication date: 2010
Journal: Journal of Molecular Recognition
Volume: 23
Issue: (5)
Page numbers: 480-485.
DOI: 10.1002/jmr.1015

Abstract: Abstract: Cyclodextrin glycosyltransferases (CGTases), members of glycoside hydrolase family 13, catalyze the conversion of amylose to cyclodextrins (CDs), circular α-(1,4)-linked glucopyranose oligosaccharides of different ring sizes. The CD containing 12 α-D-glucopyranose residues was preferentially synthesized by molecular imprinting of CGTase from Paenibacillus sp. A11 with cyclomaltododecaose (CD12) as the template molecule. The imprinted CGTase was stabilized by cross-linking of the derivatized protein. A high proportion of CD12 and larger CDs was obtained with the imprinted enzyme in an aqueous medium. The molecular imprinted CGTase showed an increased catalytic efficiency of the CD12-forming cyclization reaction, while decreased kcat/Km values of the reverse ring-opening reaction were observed. The maximum yield of CD12 was obtained when the imprinted CGTase was reacted with amylose at 40 C for 30 min. Molecular imprinting proved to be an effective means toward increase in the yield of large-ring CDs of a specific size in the biocatalytic production of these interesting novel host compounds for molecular encapsulations. Copyright 2010 John Wiley & Sons, Ltd
Template and target information: bioimprinting, cyclomaltododecaose, CD12
Author keywords: molecular imprinting, cross-linked proteins, cyclodextrin glycosyltransferase, cyclomaltododecaose, product specificity

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