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Reference type: Conference Proceeding
Authors: Yu S, Puleo DA, Luo AQ
Publication date: 2010
Article title: Preliminary study on repeatability of molecular recognition capability of a silica-based molecularly imprinted polymer.
Page numbers: Art. No. 5516486
DOI: 10.1109/ICBBE.2010.5516486

Publisher: IEEE
ISBN: 2151-7614
Conference information: 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE), 2010

Abstract: Molecular imprinting, a templated polymerization technique, is a promising method to prepare a functional polymeric network for targeted binding of molecules in a selective recognition process. In this work, a silica-based (polysiloxane) polymer was utilized in a sol-gel process to fabricate a molecularly imprinted polymer (MIP). The manufactured MIP was imprinted with a template molecule, lysozyme. To test this MIP's molecular recognitive function, the template molecule lysozyme was extracted first; then lysozyme and a competitive molecule, RNase A, were rebound; then lysozyme and RNase A were released and re-bound for a second time in order to evaluate the recognition repeatability of the MIP. Analysis of the repeated testing results implies that the template molecules' conformation and affinity played a great role in affecting the recognition accuracy and efficiency. 2010 IEEE
Template and target information: protein, lysozyme
Author keywords: lysozyme, molecular recognition, molecularly imprinted polymer, silica

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