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Reference type: Journal
Authors: Shah KA, Peoples MC, Halquist MS, Rutan SC, Karnes HT
Article Title: Microfluidic direct injection method for analysis of urinary 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) using molecularly imprinted polymers coupled on-line with LC-MS/MS.
Publication date: 2011
Journal: Journal of Pharmaceutical and Biomedical Analysis
Volume: 54
Issue: (2)
Page numbers: 368-378.
DOI: 10.1016/j.jpba.2010.09.001
Alternative URL: http://www.sciencedirect.com/science/article/B6TGX-511G1PV-1/2/58caad738e124d5f62ba6dd3752dae4c

Abstract: The work described in this paper involves development of a high-throughput on-line microfluidic sample extraction method using capillary micro-columns packed with MIP beads coupled with tandem mass spectrometry for the analysis of urinary NNAL. The method was optimized and matrix effects were evaluated and resolved. The method enabled low sample volume (200 μL) and rapid analysis of urinary NNAL by direct injection onto the microfluidic column packed with molecularly imprinted beads engineered to NNAL. The method was validated according to the FDA bioanalytical method validation guidance. The dynamic range extended from 20.0 to 2500.0 pg/mL with a percent relative error of 5.9% and a run time of 7.00 min. The lower limit of quantitation was 20.0 pg/mL. The method was used for the analysis of NNAL and NNAL-Gluc concentrations in smokers' urine
Template and target information: 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, Tobacco specific nitrosamine, NNAL, NNAL-Gluc
Author keywords: Tobacco specific nitrosamine, NNAL, molecularly imprinted polymer, On-line microfluidic solid phase extraction, LC-MS, MS

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