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Reference type: Journal
Authors: Gupta G, Singh PK, Boopathi M, Kamboj DV, Singh B, Vijayaraghavan R
Article Title: Molecularly imprinted polymer for the recognition of biological warfare agent staphylococcal enterotoxin B based on Surface Plasmon Resonance.
Publication date: 2010
Journal: Thin Solid Films
Volume: 519
Issue: (3)
Page numbers: 1115-1121.
DOI: 10.1016/j.tsf.2010.08.054
Alternative URL: http://www.sciencedirect.com/science/article/B6TW0-50VTWHJ-D/2/477928a7285cc54c5359ccc2406c807f

Abstract: Molecularly imprinted polymers (MIPs) embody a new class of materials possessing high selectivity and affinity for the target molecules. The aim of the present work is to explore the feasibility of employing the MIP as sensing material for the detection of Staphylococcal enterotoxin B (SEB) by Surface Plasmon Resonance (SPR). In this context, the MIP film was prepared by in-situ electropolymerization of 3-aminophenylboronicacid (3-APBA) on the bare gold chip in the presence of SEB molecule. The MIP of SEB exhibited a linear response from 3.2 fM to 25.6 fM (r2 = 0.99) with a detection limit of 0.05 fM. KD (equilibrium constant) and Bmax (maximum binding capacity of analyte) were calculated by using kinetic evaluation software and found to be 24 fM and 71, respectively and change in Gibb's free energy ([increment]G) value calculated using Vant's Hoff equation was found to be -77.54 kJ/mol. Interference study was performed with the homologs of SEB such as SEA and SEC in order to know the selectivity efficiency of SEB MIP and were found to be 23.57% and 23.43%, respectively
Template and target information: Staphylococcal enterotoxin B, SEB
Author keywords: Biological warfare agent, SPR, Staphylococcal enterotoxin B, Impedance, High affinity synthetic receptor


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