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Reference type: Journal
Authors: Zhao KY, Cheng GX, Wei JF, Zhou JY, Zhang JL, Chen L
Article Title: The Rebinding Properties of Bovine Serum Albumin Imprinted Calcium Alginate/Phosphate Hybrid Microspheres Via the Adjustment of pH Values and Salt Concentration.
Publication date: 2010
Journal: Macromolecular Symposia
Volume: 297
Issue: (1)
Page numbers: 126-137.
DOI: 10.1002/masy.200900105

Abstract: Abstract Summary: Bovine serum albumin imprinted calcium phosphate/alginate hydrogel microspheres were prepared with sodium alginate (SA), (NH4)2HPO4, and using CaCl2 as gelling agent, bovine serum albumin (BSA) as template in inverse suspension. The optimized rebinding properties of BSA imprinted hydrogel microspheres were investigated by controlling pH value and ionic concentration from the viewpoint of adjusting the process of gelling, removing template and rebinding. The optimized pH values for the imprinting of BSA in gelling, removing template and rebinding process was 4.1, 8.3 and 4.8, respectively. The effect of NaCl concentration on the BSA rebinding was also determined. We provided a strategy to get the optimized imprinting efficiency by altering pH value and ionic concentration in a weakly ionic cross-linked hydrogel system on the process of protein's imprinting
Template and target information: protein, bovine serum albumin, BSA
Author keywords: Bovine serum albumin, calcium phosphate, alginate hydrogel microspheres, Swelling, imprinting efficiency, pH value, molecular imprinting

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