MIPdatabase
Authors: Zhao KY, Cheng GX, Wei JF, Zhou JY, Zhang JL, Chen L
Article Title: The Rebinding Properties of Bovine Serum Albumin Imprinted Calcium Alginate/Phosphate Hybrid Microspheres Via the Adjustment of pH Values and Salt Concentration.
Publication date: 2010
Journal: Macromolecular Symposia
Volume: 297
Issue: (1)
Page numbers: 126-137.
DOI: 10.1002/masy.200900105
Abstract: Abstract Summary: Bovine serum albumin imprinted calcium phosphate/alginate hydrogel microspheres were prepared with sodium alginate (SA), (NH4)2HPO4, and using CaCl2 as gelling agent, bovine serum albumin (BSA) as template in inverse suspension. The optimized rebinding properties of BSA imprinted hydrogel microspheres were investigated by controlling pH value and ionic concentration from the viewpoint of adjusting the process of gelling, removing template and rebinding. The optimized pH values for the imprinting of BSA in gelling, removing template and rebinding process was 4.1, 8.3 and 4.8, respectively. The effect of NaCl concentration on the BSA rebinding was also determined. We provided a strategy to get the optimized imprinting efficiency by altering pH value and ionic concentration in a weakly ionic cross-linked hydrogel system on the process of protein's imprinting
Template and target information: protein, bovine serum albumin, BSA
Author keywords: Bovine serum albumin, calcium phosphate, alginate hydrogel microspheres, Swelling, imprinting efficiency, pH value, molecular imprinting
Join the Society for Molecular Imprinting
New items RSS feed
View latest updatesSign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.
Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
