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Reference type: Journal
Authors: Lemay P
Article Title: The use of high pressure for separation and production of bioactive molecules.
Publication date: 2002
Journal: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Volume: 1595
Issue: (1-2)
Page numbers: 357-366.
DOI: 10.1016/S0167-4838(01)00356-9

Abstract: Due to its action on the forces governing inter- and intramolecular interactions, the application of high pressure to biopurification or bio-elaboration of a product are of interest. The two closely thermodynamically related parameters. pressure and temperature, render processes based on their action clean, as no chemical reagents have to be added (and thus further removed) when they are applied. The use of high pressure in the development of desorption methods for the purification of bioactive molecules, particularly in the immunoaffinity field, is reviewed and discussed. Also mentioned is the application of the pressure parameter during the synthesis of a bioreagent. Finally, integrated processes relative to the synthesis and purification of these compounds are proposed. (C) 2002 Elsevier Science B.V. All rights reserved


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