Abstract: We have studied the influence of the experimental conditions during synthesis on the properties of molecularly imprinted sol-gels for serine. The influence of hydrophobicity, presence of amino groups, additives, water/ethanol ratio and type of catalyst (basic or acid) were optimized using a Plackett-Burmann design in 12 blocks. Serine was selected as a target molecule for the imprinting process, and each imprinted and non-imprinted material was studied by competitive and non-competitive protocols. The results demonstrate the strong effect of 3-aminopropyltrimethoxysilane on the recognition capabilities of the materials in competitive assays. The mechanisms of interaction appear to be similar in acid-catalysed sol-gels, whereas sol-gels obtained under base catalysis tend to give interactions affecting the competitive experiments only.
Template and target information: serine
Author keywords: molecular imprinting, MIP, Serine, Plackett-Burman