MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Wang HF, Yang H, Zhang LM
Article Title: Temperature-sensitive molecularly imprinted microgels with esterase activity.
Publication date: 2011
Journal: SCIENCE CHINA Chemistry
Volume: 54
Issue: (3)
Page numbers: 515-520.
DOI: 10.1007/s11426-010-4200-z
Alternative URL: http://chem.scichina.com:8081/sciBe/EN/abstract/abstract502284.shtml

Abstract: Temperature-sensitive molecularly imprinted microgels (MIGs) exhibiting esterase activity were prepared by a reverse emulsion method using dialdehyde dextran-histidine conjugate (PAD-His) as the functional macromonomer and p-nitrophenyl phosphate (NPP) as the stable transition state analogue (TSA) as well as Co2+ as the coordination center. The catalytic activity of MIGs was greatly influenced by the amount of the template, and could be modulated by temperature. The hydrolysis kinetics of p-nitrophenyl acetate (NPA) in the presence of MIGs could be described by the Michaelis-Menten equation. The Michaelis-Menten constant and maximum velocity were found to be 2.2 x 10 -5 mol/L and 2.04 x 10 8 mol/h, respectively. In addition, the MIGs were found to have a high catalytic selectivity to NPA
Template and target information: p-nitrophenyl phosphate, NPP, transition state analogue, TSA, p-nitrophenyl acetate, NPA
Author keywords: molecular imprinting, microgels, esterase, temperature sensitivity, dextran

  Periodic table Trainer shirt  MIPs peptide mug  Gravity waves shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner