MIPdatabase
Authors: Shen N, Yan F, Guo Y, Lu SW, Gong PS, Xu YW, Yan GL, Mu Y, Luo GM
Article Title: Imprinted Human Serum Albumin with Antioxidant Activity.
Publication date: 2011
Journal: Chemical Research in Chinese Universities
Volume: 27
Issue: (2)
Page numbers: 258-263.
Alternative URL: http://www.cjcu.jlu.edu.cn/hxyj/EN/article/downloadArticleFile.do?attachType=PDF&id=13223
Abstract: In order to create a new mimic of glutathione peroxidase(GPx), bioimprinting was used to generate glutathione(GSH) binding site and chemical modification was used to incorporate catalytic group selenocystine(Sec). Human serum albumin(HSA) and S-substituted dinitrophenyl glutathione(GSH-S-DNP) were chosen as the imprinted matrix and imprinting template, respectively, to generate a GSH-imprinted protein(GSH-HSA) by bioimprinting. Sec was incorporated into the GSH-HSA by chemical modification to give a new GPx mimic(Se-GSH-HSA). Se-GSH-HSA displayed considerably higher GPx activity than non-printed HSA(Se-HSA). The enzymic properties and kinetics of Se-GSH-HSA were studied. Moreover, Se-GSH-HSA was confirmed to have stronger antioxidant ability to protect mitochondria against oxidative damage with ferrous sulfate/ascorbate-induced mitochondria damage model, indicating that Se-GSH-HSA has potential application in medicine.
Template and target information: bioimprinting, glutathione, GSH
Author keywords: antioxidant, Enzyme mimic, glutathione peroxidase, molecular imprinting
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