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Reference type: Journal
Authors: Kowalski D, Pobozy E, Trojanowicz M
Article Title: Flow-Injection Preconcentration of Chloramphenicol Using Molecularly Imprinted Polymer for HPLC Determination in Environmental Samples.
Publication date: 2011
Journal: Journal of Automated Methods and Management in Chemistry
Volume: 2011
Page numbers: Art. No. 143416.
DOI: 10.1155/2011/143416
Alternative URL: http://downloads.hindawi.com/journals/jammc/2011/143416.pdf

Abstract: The residue of antibiotic chloramphenicol (CAP) is important issue for food quality control and also for the environmental monitoring. It is banned for use in food-producing animals and has very limited use in human medicine, because of its severe impact on human health. Determination of trace level of CAP in environmental samples requires a very sensitive analytical method and efficient preconcentration procedure. CAP can be efficiently preconcentrated in flow-injection system using flow-through reactor packed with molecularly imprinted polymer (MIP), but determination of CAP in eluate from MIP requires the application of chromatographic separation, which was made in reversed-phase HPLC system with UV detection. In optimized conditions the limit of detection for 100 mL sample in HPLC with offline preconcentration on MIP was evaluated as 0.66 mg/L. In hyphenated FIA-HPLC system with zone sampling the LOD for developed method was evaluated as 15 ng/L, which indicates the possibility of using it for analysis of environmental samples.
Template and target information: chloramphenicol, CAP

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