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Reference type: Journal
Authors: Khorrami AR, Taherkhani M
Article Title: Synthesis and Evaluation of a Molecularly Imprinted Polymer for Pre-concentration of Patulin from Apple Juice.
Publication date: 2011
Journal: Chromatographia
Volume: 73
Issue: (Suppl. 1)
Page numbers: S151-S156.
DOI: 10.1007/s10337-010-1892-3
Alternative URL: http://www.springerlink.com/content/f727140v47r0804q/

Abstract: Patulin, (4-hydroxy-4H-furo[3,2-c] pyran-2(6H)-one) is a toxic secondary metabolite produced by a wide range of fungal species growing on some fruits, including apples. A novel molecularly imprinted polymer (MIP) for patulin has been synthesized using oxindole as a dummy template. The synthesis of MIPs based on dummy templates is a solution to overcome template bleeding shortcoming in trace analysis. The polymer was prepared in a non-covalent approach with methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linker via free radical polymerization. It was revealed that the MIP particles in extraction columns displayed high affinity towards patulin and, therefore, subsequently employed for molecularly imprinted-solid phase extraction (MIP-SPE) of patulin from contaminated apple juice samples. The analysis of spiked samples showed good recoveries (>80%). Reproducibility, repeatability, and limit of detection of the proposed method were also investigated
Template and target information: oxindole, patulin, 4-hydroxy-4H-furo[3,2-c] pyran-2(6H)-one
Author keywords: column liquid chromatography, molecularly imprinted polymer, solid phase extraction, Oxindole, patulin, apple juice

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