Abstract: Molecularly imprinted polymer microspheres (MIPMs) were prepared by precipitation polymerization of methacrylic acid (MAA) and ethylene dimethacrylate (EDMA) using cholesterol (CHO) as template molecule in toluene-acetonitrile mixed solvent (9:1, j). The MIPMs were investigated in equilibrium binding experiment and Scatchard analysis to evaluate the binding characteristics and recognition mechanism of MIPMs in aqueous solution. Scatchard analysis showed that two classes of binding sites were produced in the polymer matrix under the studied concentration. Their dissociation constant (Kd) and apparent maximum binding capacity (Qmax) were 0.86 mmol/L, and 80.4 μmol/g for high affinity binding sites and 0.39 mmol/L and 61.6 μmol/g for low affinity binding sites. The CHO imprinted polymer micro-spheres synthesized in this method had better performance and could be used for separation, enrichment and analysis of CHO.
Template and target information: cholesterol, CHO
Author keywords: cholesterol, Molecularly imprinted polymer microspheres, precipitation polymerization