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Reference type: Conference Proceeding
Authors: Moein MM, Javanbakht M, Akbari-Adergani B
Publication date: 2011
Article title: Preparation a Monolithic loop by molecularly imprinted polymers for on-line extraction and determination of Tramadol in human plasma and urine with high performance liquid chromatography.
Alternative URL: http://icc2011.ir/Docs/D.pdf

Conference information: Abstracts, 15th Iranian Chemistry Congress

Abstract: In this paper, a novel solid phase extraction (SPE) method for the determination of Tramadol (TRD) in plasma by molecularly imprinted monolithic loop was developed. Using tremadol as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker andchloroform as the solvent, a tremadol imprinted monolithic loop was prepared by in situ molecular imprinting technique for the first time, and the optimal synthesis conditions and the selectivity of TRD imprinted monolithic loop were investigated. The high selectivity of the sorbent in an on-line molecularly imprinted monolithic HPLC loop permitted a simple and rapid analysis of this drug inurine samples showed with limits of detection (LOD) and quantification (LOQ) of 0.33 ng mL-1 and 0.10 ng mL-1, respectively. These limits for tramadol in plasma samples were 1.1 and 3.3 ng mL-1, respectively
Template and target information: tramadol, TRD

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