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Reference type: Journal
Authors: Mohajeri SA, Hosseinzadeh H, Keyhanfar F, Aghamohammadian J
Article Title: Extraction of crocin from saffron (Crocus sativus) using molecularly imprinted polymer solid-phase extraction.
Publication date: 2011
Journal: Clinical Biochemistry
Volume: 44
Issue: (13, Supplement 1)
Page numbers: S136.
DOI: 10.1016/j.clinbiochem.2011.08.326
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0009912011017826

Abstract: A new molecularly imprinted polymer for extraction of crocin from saffron stigmas was prepared using gentiobiose (a glycoside moiety in crocin structure) as a template. Crocin binding to gentiobiose imprinted polymer (Gent-MIP) was studied in comparison with a blank nonimprinted polymer in aqueous media. Affinity of the Gent-MIP for the crocin was more than the nonimprinted polymer at all concentrations. In Scatchard analysis, the number of binding sites in each gram of polymer (maximum binding sites) and dissociation constant of crocin to binding sites were 18.4 mmol/g polymer and 11.2 mM, respectively. The Gent-MIP was then used as the sorbent in an SPE method for isolation and purification of crocin from methanolic extract of saffron stigmas. The recovery of crocin, safranal and picrocrocin was determined in washing and elution steps. The Gent-MIP had significantly higher affinity for crocin than other compounds and enabled selective extraction of crocin with a high recovery (84%) from a complex mixture. The results demonstrated the possibility of using a part of a big molecule in preparing a molecularly imprinted polymer with a good selectivity for the main structure.
Template and target information: gentiobiose, Gent, crocin
Author keywords: Saffron, Crocin, molecularly imprinted polymer, Gentibiosis, HPLC

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