MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Harz S, Schimmelpfennig M, Tse Sum Bui B, Marchyk N, Haupt K, Feller KH
Article Title: Fluorescence optical spectrally resolved sensor based on molecularly imprinted polymers and microfluidics.
Publication date: 2011
Journal: Engineering in Life Sciences
Volume: 11
Issue: (6)
Page numbers: 559-565.
DOI: 10.1002/elsc.201000222

Abstract: Abstract: Molecularly imprinted polymers (MIPs) are synthetic receptors that can be synthesized for a variety of target molecules. One of their possible applications is in chemical sensors. In this article, a microfluidic measurement setup with immobilized MIPs with fluorescence detection is described. Fluorescence measurements were performed on MIP films specific for the fluorescence-labeled amino acid dansyl-L-phenylalanine. A conventional imprinting protocol with noncovalent interactions between the functional monomers and the template was used. We present the immobilization of the polymers on quartz substrates to form homogenous and stable films over at least six cycles of measurement and regeneration, and their use for microfluidic measurements in a spectrofluorometer. Furthermore, the design and buildup for a reproducible fluorescence measuring system is described. With this system, concentrations of 0.5 μM of dansyl-L-phenylalanine could be detected with 3 sigma certainty which is 50 times more sensitive compared with the detection of dansyl-L-phenylalanine without MIPs under the same conditions. The binding kinetics indicates that binding is completed after 30 min. Our results show that MIP films in combination with fluorescence detection and microfluidic devices can be a very sensitive tool for the detection of biologically relevant analytes
Template and target information: dansyl-L-phenylalanine
Author keywords: biosensor, Dansyl-L-phenylalanine, fluorescence, Microfluidics, Molecularly imprinted polymers


  Shirt featuring the name Karsten spelled out in symbols of the chemical elements  Mug featuring the name Karsten spelled out in the single letter amino acid code  Meerkat somebody mention coffee mug

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner