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Reference type: Journal
Authors: Schirhagl R, Hall EW, Fuereder I, Zare RN
Article Title: Separation of bacteria with imprinted polymeric films.
Publication date: 2012
Journal: Analyst
Volume: 137
Issue: (6)
Page numbers: 1495-1499.
DOI: 10.1039/C2AN15927A
Alternative URL: https://www.researchgate.net/publication/221822535_Separation_of_bacteria_with_imprinted_polymeric_films

Abstract: Separation of compounds out of complex mixtures is a key issue that has been solved for small molecules by chromatography. However, general methods for the separation of large bio-particles, such as cells, are still challenging. We demonstrate integration of imprinted polymeric films (IPF) into a microfluidic chip, which preferentially capture cells matching an imprint template, and separate strains of cyanobacteria with 80-90% efficiency, despite a minimal difference in morphology and fluorescence, demonstrating its general nature. It is currently thought that the imprinting process, conducted while the polymer cures, transfers chemical information of the cell's external structure to the substrate. Capture specificity and separation can be further enhanced by orienting the imprints parallel to the flow vector and tuning the pH to a lower range
Template and target information: bacteria, cyanobacteria

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