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Reference type: Journal
Authors: Taguchi Y, Takano E, Takeuchi T
Article Title: SPR Sensing of Bisphenol A Using Molecularly Imprinted Nanoparticles Immobilized on Slab Optical Waveguide with Consecutive Parallel Au and Ag Deposition Bands Coexistent with Bisphenol A-Immobilized Au Nanoparticles.
Publication date: 2012
Journal: Langmuir
Volume: 28
Issue: (17)
Page numbers: 7083-7088.
DOI: 10.1021/la300018t

Abstract: A slab-type optical waveguide (s_OWG)-based microfluidic SPR measurement system for bisphenol A was developed. This s_OWG possesses consecutive parallel gold and silver deposition bands in the line of plasmon flow, allowing two individual SPR signals to be independently obtained as a result of the difference in resonant reflection spectra of these metals. As a molecular recognition element, molecularly imprinted polymer nanoparticles (MIP-Np) were employed and immobilized on the surface of each of the gold and silver deposition bands. The resonant reflection spectra were measured on the MIP-Np-immobilized consecutive parallel gold and silver deposition bands coexistent with BPA-AuNp. The Ag-based SPR spectra showed a red shift (0.7 nm) when free BPA (0.1 mM) was passed over the BPA-AuNp/immobilized MIP-Np complexes formed on the s_OWG, unlike the case for the Au deposition band, while a large excess of BPA induced a blue shift due to the competitive desorption of BPA-AuNp from the immobilized MIP-Np on the s_OWG. By using the proposed detection system, binding events of other small molecules could be monitored in conjunction with the use of MIP-Np and labeled-AuNp.
Template and target information: bisphenol A, BPA


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