Abstract: A molecularly imprinted film sensor was prepared by cyclic voltammetry on a gold electrode surface using O-aminophenol as the monomer, microcystin (MC-LR) as a template, and then MC-LR was determined by amperometry. The optimum preparation conditions for the sensor were as follows: pH = 4.5, monomer/template = 1.4 x 108 : 1; elution time = 10 min. The characteristics of the sensor such as the linear relationship, service life, selectivity were investigated. Experimental results showed that the linear response of the sensor is 0.05-0.35 mg/L, the recovery of standard addition is 80% to 105% and the detection limit is 7.3 μg/L. In comparison with HPLC, when the confidence level was 99%, there is no systematic error. The sensor exhibited a good selectivity and sensitivity.
Template and target information: microcystin-LR, MC-LR
Author keywords: microcystins, molecular imprinting, Electricity polymerization, sensor