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Reference type: Journal
Authors: Lulinski P, Maciejewska D
Article Title: Effective separation of dopamine from bananas on 2-(3,4-dimethoxyphenyl)ethylamine imprinted polymer.
Publication date: 2012
Journal: Journal of Separation Science
Volume: 35
Issue: (8)
Page numbers: 1050-1057.
DOI: 10.1002/jssc.201100910

Abstract: A 2-(3,4-dimethoxyphenyl)ethylamine imprinted polymer (MIPpt) was prepared via the precipitation polymerization together with a nonimprinted polymer (NIP). The morphology of particles was investigated by scanning electron microscopy and the specific surface areas were estimated by methylene blue adsorption (60.5 3.5 and 36.9 1.2 m2/g for MIPpt and NIP, respectively). The binding experiments were performed to determine the binding capacity of MIPpt/NIP particles toward dopamine. Next, the effects of solvents on loading, washing, and eluting steps were examined on solid-phase extraction (SPE). Methanol-water 85:15 v/v (loading step), methanol (washing step), and 0.04 M aqueous ammonium acetate-methanol 30:70 v/v (eluting step) were selected as the most effective systems. Described SPE protocol was successfully applied for separation of dopamine on 2-(3,4-dimethoxyphenyl)ethylamine imprinted particles. Finally, the molecularly imprinted polymer was used for determination of dopamine in spiked banana extract. The total recovery of dopamine from MIPpt was equal to 88.5 4.6%, but from NIP was only 12.8 2.3%. The developed material and method were demonstrated to be applicable for the separation of dopamine from bananas. The commercial sorbent C18 was not suitable to such application
Template and target information: 2-(3,4-dimethoxyphenyl)ethylamine, dopamine
Author keywords: Banana sample, dopamine, Molecularly imprinted polymers, Solid-phase extraction


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