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Abstract: Molecular imprinted polymer microspheres were synthesized by aqueous suspension polymerization using polyvinyl alcohol 1735(PVA) as dispersive reagent, rifampicin (RFP) as template molecule,MAA as functional monomers and EGDMA as the crosslinker with high selectivity for RFP.The polymers were investigated in equilibrium binding experiments and Scatchard analysis to evaluate the binding characteristics and recognition mechanism of the RFP-molecular imprinted polymer in aqueous solution.Scatchard analysis showed that two binding sites were formed in the imprinted polymer matrix under the studied concentrations.The dissociation constant(Kd1) and the apparent maximum binding capacity(Qmax1) were Kd1 = 1.08 x 10-2 mg/mL, Qmax1 = 14.40 mg/g for high affinity binding sites and Kd2 = 7.18 x 10-3 mg/mL, Qmax2 = 12.51 mg/g for low affinity binding sites. The experiments of binding different substrates showed that the imprinted polymer possessed a high selectivity and a high recognition ability for RFP.The method has advantages of simpler operation and higher accuracy,and may be widely used.
Template and target information: rifampicin, RFP
Author keywords: molecularly imprinted microspheres, molecular recognition mechanism, aqueous micro-suspension polymerization, rifampicin