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Reference type: Journal
Authors: Çorman ME, Akgöl S
Article Title: Preparation of molecular imprinted hydrophobic polymeric nanoparticles having structural memories for lysozyme recognition.
Publication date: 2012
Journal: Artificial Cells, Blood Substitutes and Biotechnology
Volume: 40
Issue: (4)
Page numbers: 245-255.
DOI: 10.3109/10731199.2012.657204

Abstract: This study is related to the preparation of lysozyme-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tyroptophan methylester) [nano-MIP] nanoparticles for purification of lysozyme. Nano-MIP particles were found to be 261 nm in diameter with a surface area of 1648 m2/g. According to the elemental analysis results, the particles contained 0.85 μmol MATrp/g polymer. The maximum lysozyme adsorption capacity was 1182.8 mg/g. Adsorbed lysozyme was desorbed with 94% recovery. It was observed that after five adsorption-desorption cycles there was no significant loss in adsorption capacity. In order to show the selectivity of the Lys-MIP nanoparticles, adsorption of lysozyme, bovine serum albumin (BSA), and cytochrome c were investigated
Template and target information: protein, lysozyme, lys
Author keywords: molecular imprinted polymers, lysozyme, affinity recognition, nanoparticles, selective protein adsorption

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