MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Huang S, Nilsson PH, Sandholm K, Elmlund L, Nicholls IA, Ekdahl KN
Article Title: Regulation of complement in whole blood by heparin molecularly imprinted polymer particles.
Publication date: 2012
Journal: Immunobiology
Volume: 217
Issue: (11)
Page numbers: 1199.
DOI: 10.1016/j.imbio.2012.08.200
Alternative URL: http://www.sciencedirect.com/science/article/pii/S017129851200397X

Abstract: Synthetic materials mimicking biomolecular structure and function provide an attractive alternative to the use of materials of biological origin for a range of biomaterials and diagnostic applications. The aim of this study is to make molecular imprints of heparin on polymer surfaces in order to attenuate surface-induced complement activation. Molecularly imprinted polymers (MIPs) were prepared by polymerizing various mixtures of monomers in the presence of heparin. The resulting polymers were ground to particles and the bound heparin extracted, leaving cavities complementary to the structure of heparin in the polymers. We compared the MIPs and non-imprinted reference polymer particles regarding complement activation (C3a, sC5b-9) and leukocyte activation (CD11b expression, cytokine release) in lepirudin blood with polystyrene (PS) particles as a control. In addition, adsorption of complement proteins (C1q, properdin, C3, C4, C5, and C4 binding protein; C4BP) to the polymer surfaces was measured by an enzyme immunoassay. The MIPs induced significantly lower complement and cellular activation compared to the reference polymers. This was associated with lower binding of C3, C4 and C5, and a higher ratio between C4BP and C4 for the MIP, while both polymers bound similar amounts of the recognition molecules C1q and properdin. Preincubation of MIP and reference polymers with soluble heparin did not affect any of the measured parameters, indicating that the imprint in the polymer surface was directly responsible for the biological activity. Our study demonstrates that heparin molecularly imprinted polymer particles could significantly attenuate complement and leukocyte activation, offering potential for developing auto-regulating surfaces.
Template and target information: heparin

  mipdatabase.com logo imprinters do it in bulk shirt  Mug featuring the name Ian spelled out in the single letter amino acid code  Perpetual student shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner