Abstract: A highly selective molecularly imprinted electrochemical sensor of uric acid (UA) was prepared with chitosan (CS) as functional matrix and uric acid as template molecule via constant potential electrochemical deposition. The imprinted membrane was characterized by Attenuated total reflection infrared spectroscopy (ATR-FTIR) and Electrochemical impedance spectroscopy (EIS); the performances of the sensor were studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The UA had good electrochemical response in 0.1 mol L-1 phosphate buffer solution (PBS) (pH=5.0). The oxidation peak current of DPV was well-proportional to the concentration of UA in the range from 0.1 μmol L-1 to 80.0 μmol L-1, with a correlation coefficient of 0.999 1. The developed sensor exhibited specific recognition for UA against the competitors which consisted of similar structure and coexisting interference in human serum. Moreover, the sensor also shows exellent reproducibility and was successfully employed to the determination of UA in human serum with the recovery of 97.41%~102.8%.
Template and target information: uric acid, UA
Author keywords: uric acid, Electrochemical sensor, chitosan, molecularly imprinted