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Reference type: Journal
Authors: Yan HY, Cheng XL, Sun N, Cai TY, Wu RJ, Han K
Article Title: Rapid and selective screening of melamine in bovine milk using molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography-ultraviolet detection.
Publication date: 2012
Journal: Journal of Chromatography B
Volume: 908
Page numbers: 137-142.
DOI: 10.1016/j.jchromb.2012.09.022
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1570023212005521

Abstract: A simple, convenient and high selective molecularly imprinted matrix solid-phase dispersion (MI-MSPD) using water-compatible cyromazine-imprinted polymer as adsorbent was proposed for the rapid screening of melamine from bovine milk coupled with liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized by cyromazine as dummy template and reformative methanol-water system as reaction medium showed higher affinity and selectivity to melamine, and so they were applied as the specific dispersant of MSPD to extraction of melamine and simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.24 - 60.0 μg g-1 with the correlation coefficient of 0.9994. The recoveries of melamine at three spiked levels were ranged from 86.0 to 96.2% with the relative standard deviation (RSD) 4.0%. This proposed MI-MSPD method combined the advantages of MSPD and MIPs, and could be used as an alternative tool for analyzing the residues of melamine in complex milk samples
Template and target information: melamine
Author keywords: Matrix solid-phase dispersion, Dummy imprinted polymers, Melamine, high performance liquid chromatography, Milk samples

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