MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Santos MG, Vitor RV, Andrade FL, Martins I, Figueiredo EC
Article Title: Molecularly imprinted solid phase extraction of urinary diethyl thiophosphate and diethyl dithiophosphate and their analysis by gas chromatography-mass spectrometry.
Publication date: 2012
Journal: Journal of Chromatography B
Volume: 909
Page numbers: 70-76.
DOI: 10.1016/j.jchromb.2012.10.015
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1570023212005995

Abstract: An analytical method involving molecularly imprinted solid phase extraction (MISPE) and gas chromatography-mass spectrometry (GC-MS) was developed for the analysis of organophosphates metabolites (diethyl thiophosphate - DETP and diethyl dithiophosphate - DEDTP) in human urine samples. A DETP molecularly imprinted polymer (MIP) was synthesized using 4-vinylpiridine as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. The conditioning step of the MISPE was conducted by running 3 mL of acetonitrile, 3 mL of 0.1 mol L-1 dibasic phosphate buffer at pH 11 and 2 mL of water through the molecularly imprinted polymer (MIP) cartridge. The extraction step was executed using 1.0 mL of a urine sample, with the pH previously adjusted to 3.0. Finally, the analytes were eluted with 3 mL of acetonitrile and derivatized with 3% 2,3,4,5,6-pentafluorobenzyl bromide solution at room temperature for 1 h. The sample was analyzed by GC-MS in the SIM (selected ion monitoring) mode. Analytical calibration curves for DETP and DEDTP were constructed using a pool of urine samples and six levels of concentration. The method was found to be linear from 10 to 500 μg L-1 (r > 0.99) with limits of quantification of 10 μg L-1 for both analytes. The within-day and between-day precisions were evaluated (as %RSD) and all the results were < 15% for both analytes. The method was accurate (relative error < 15%), with good robustness
Template and target information: diethyl thiophosphate, DETP, diethyl dithiophosphate, DEDTP
Author keywords: MIP, Dialkyl phosphates, SPE, Pentafluorobenzyl bromide, Gas chromatography-mass spectrometry, Organophosphates

  mipdatabase.com logo imprinters do it in bulk shirt  Science Teacher peptide mug  Perpetual student shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner