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Reference type: Journal
Authors: Chen TJ, Zhao JC, Guo ZA, Zhang BX
Article Title: Grafting of doxycycline imprinted polymers on silica surface for selective solid-phase extraction in beef samples.
Publication date: 2012
Journal: Analytical Methods
Volume: 4
Issue: (12)
Page numbers: 4237-4243.
DOI: 10.1039/C2AY26084K

Abstract: A molecularly imprinted polymer (MIP) for separation and enrichment of doxycycline (DC) has been synthesized using DC as the template, acrylamide-tung oil acid methyl ester (AM-ESA) and propenoic acid (AA) as the functional monomers, ethylene glycol dimethacrylate (EGDMA) as the cross-linker and 2,2'-azo-bis-isobutyronitrile (AIBN) as the initiator. The chemical and morphological characteristics of the MIPs were investigated by FT-IR and SEM. The static and kinetic adsorption, selectivity and recovery of the polymers were evaluated. The results indicated that the MIPs had higher chemical affinity for DC than the non-imprinted polymer (NIP). The maximum adsorption capacity of the DC-MIPs was calculated to be 257 μmol g-1. Finally, the MIPs were successfully used as a stationary phase for solid-phase extraction (SPE) to enrich DC from beef samples and the recoveries were in the range of 92.5-96.4% with RSDs of 3.1-7.0%
Template and target information: doxycycline, DC

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