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Reference type: Journal
Authors: Ying XG, Qi LL, Li X, Zhang WY, Cheng GX
Article Title: Stimuli-responsive recognition of BSA-imprinted poly vinyl acetate grafted calcium alginate core-shell hydrogel microspheres.
Publication date: 2013
Journal: Journal of Applied Polymer Science
Volume: 127
Issue: (5)
Page numbers: 3898-3909.
DOI: 10.1002/app.37730

Abstract: Poly vinyl acetate grafted calcium alginate hydrogel microspheres were prepared with bovine serum albumin (BSA) as molecular template. The microspheres exhibited homogeneous and core-shell structure according to different preparation strategy. The rebinding and swelling property of microspheres showed responsiveness toward ionic strength, temperature, and pH. It was found the highest separation factor of 1.85 and imprinting efficiency of 1.75 when the Ca2+ ionic strength was 1.25 and 0.34 mol/kg, respectively. The separation factor was found decreased as temperature grew from 29 to 45 C while the imprinting efficiency reached a peak value at about 37 C. Separation factor of BSA imprinted microspheres at different pH was also recorded and two peaks were found, which were considered to be caused by the similar swollen state due to ionic and covalent crosslinking structure of modified microspheres. The recognition responsiveness is suggested to be influenced by environmental effects due to the changing in imprints' configuration at different swollen states. 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Template and target information: protein, bovine serum albumin, BSA
Author keywords: molecular imprinting, proteins, modification, core-shell polymers, hydrogels


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