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Reference type: Journal
Authors: Ugajin H, Oka N, Okamoto T, Kawaguchi H
Article Title: Polymer particles having molecule-imprinted skin layer.
Publication date: 2013
Journal: Colloid & Polymer Science
Volume: 291
Issue: (1)
Page numbers: 109-115.
DOI: 10.1007/s00396-012-2685-z

Abstract: We developed a novel type of protein molecule-imprinted particles. Bovine hemoglobin (BHb) and submicron-sized polymer particles were used in this study as a template and a substrate, respectively. BHb molecules were softly and sparsely adsorbed on the particle surface. Then skin layer was formed with a thickness of BHb molecule size by living radical graft polymerization of moderately hydrophilic monomers. After that, BHb molecules were removed from their embedded spaces. The ability of vacant spaces on the surface layer of particles to take BHb molecules selectively was examined. Successful results were obtained not only in single protein adsorption system but also in the competitive adsorption system of several proteins and BHb, that is, the imprinted space was reserved for BHb even if other proteins coexisted, although their nonspecific adsorption on non-imprinted surface was not avoided
Template and target information: protein, bovine hemoglobin, BHb
Author keywords: molecule imprinting, Skin layer, microsphere, hemoglobin


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