MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Lv YK, Jia CL, Zhang JQ, Li P, Sun HW
Article Title: Preparation and characterization of molecularly imprinted monolithic column for on-line solid-phase extraction coupled with hplc for determination of the fluoroquinolone residues in honey samples.
Publication date: 2013
Journal: Journal of Liquid Chromatography & Related Technologies
Volume: 36
Issue: (2)
Page numbers: 249-260.
DOI: 10.1080/10826076.2011.649877

Abstract: A new ofloxacin-imprinted monolithic precolumn on-line coupling with reversed-phase high performance liquid chromatography was developed for the determination of the fluoroquinolone residues in honey samples. The molecularly imprinted monolithic column (MIP-MC) was prepared by in situ polymerization in a stainless steel chromatographic column, using methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linker, and chloroform and dodecanol as the mixed porogenic solvents. The optimal synthesis conditions and on-line extraction conditions were investigated. The maximum binding capacity (51.50 mg/g) and the dynamic binding capacity (434.92 μg/mL) of the MIP-MC were evaluated by Scatchard analysis and the breakthrough curves, respectively, and they are higher than the non-imprinted monolithic column (43.15 mg/g and 96.52 μg/mL). An enrichment factor of 15.4 along with a good sample cleanup effect was obtained under the optimized conditions. The recoveries of the method for three fluoroquinolones such as ofloxacin, ciprofloxacin, and enrofloxacin were investigated, and high recoveries of 82.0 - 90.2% from honey samples were obtained with precision between 2.5% and 6.9%. The limits of detection and quantitation of the proposed method were in a range of 5.68 - 9.71 (μg/kg and 18.93 - 32.37 (μg/kg, respectively. The method was successfully applied to on-line preconcentration, separation, and determination of the fluoroquinolone residues in food
Template and target information: ofloxacin
Author keywords: Fluoroquinolones, Honey, Molecularly imprinted polymers, monolithic column, Ofloxacin, on-line, Solid-phase extraction


  I love MIPs bag  multi MIPs logo mug  British periodic table patriotic tote bag

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner