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Reference type: Journal
Authors: Wang XD, Dong J, Ming HM, Ai SY
Article Title: Sensing of glycoprotein via a biomimetic sensor based on molecularly imprinted polymers and graphene-Au nanoparticles.
Publication date: 2013
Journal: Analyst
Volume: 138
Issue: (4)
Page numbers: 1219-1225.
DOI: 10.1039/C2AN36297J

Abstract: A novel strategy was proposed for preparing a highly sensitive glycoprotein sensor based on molecularly imprinted polymers (MIP), which was electropolymerized with o-phenylenediamine and 3-aminophenylboronic acid monohydrate in the presence of template molecules (bovine serum albumin (BSA)). Sensitivity improved dramatically owing to the application of a graphene-Au nanoparticles hybrid as the electrode modifier, and the immobilization of a large amount of 6-ferrocenylhexanethiol, as the electroactive species, onto nanoparticles. The quantification of BSA was realized by detecting the electrochemical oxidation signal of 6-ferrocenylhexanethiol, which was bonded onto the electrode. Under optimized conditions, a good relationship was obtained between the response current and logarithm of BSA concentration in the range of 1.0 x 10-11 to 1.0 x 10-5 g mL-1 with a detection limit of 7.5 x 10-12 g mL-1 (S/N = 3). The resulting MIP sensor displayed good selectivity, reproducibility and stability
Template and target information: glycoprotein, bovine serum albumin, BSA

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