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Reference type: Journal
Authors: Que XH, Liu BQ, Fu LB, Zhuang JY, Chen GN, Tang DP
Article Title: Molecular Imprint for Electrochemical Detection of Streptomycin Residues Using Enzyme Signal Amplification.
Publication date: 2013
Journal: Electroanalysis
Volume: 25
Issue: (2)
Page numbers: 531-537.
DOI: 10.1002/elan.201200468
Alternative URL: https://www.researchgate.net/publication/262903454_Molecular_Imprint_for_Electrochemical_Detection_of_Streptomycin_Residues_Using_Enzyme_Signal_Amplification

Abstract: We have designed a new molecularly imprinted co-polymer (MIP) for the sensitive detection of streptomycin (STR) in food using enzymes as signal amplification. The MIP was fabricated via co-polymerization of aniline and o-phenylenediamine on gold substrate in the presence of STR as template. The assay is based on competitive binding of free STR and glucose oxidase-labeled STR (GOx-STR) to the imprinters on the MIP. On addition of glucose, hydrogen peroxide is formed that is detected by differential pulse voltammetry. Under optimal conditions, the decrease of the catalytic current is proportional to the STR concentration in the range from 0.01 to 10 ng mL-1, with a detection limit (LOD) of 7.0 pg mL-1 STR (at 3sB). Intra- and inter-assay coefficients of variation (CVs) are < 10.5 %. The system was further validated and evaluated with STR-spiked samples including honey and milk, and the recovery was between 82 and 124.2 %
Template and target information: steptomycin, STR
Author keywords: Molecularly imprinted polymers, electrochemistry, enzymatic catalysis, signal amplification, Streptomycin residue


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