MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Xu L, Hu YF, Shen F, Li QS, Ren XQ
Article Title: Specific recognition of tyrosine-phosphorylated peptides by epitope imprinting of phenylphosphonic acid.
Publication date: 2013
Journal: Journal of Chromatography A
Volume: 1293
Page numbers: 85-91.
DOI: 10.1016/j.chroma.2013.04.013
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0021967313005918

Abstract: Protein phosphorylation on tyrosine residues is an important cell signaling mechanism that regulates embryo development, cell growth, differentiation and migration. Here, we developed an epitope imprinting approach to selectively enrich tyrosine-phosphorylated (pTyr) peptides using molecularly imprinted polymers (MIPs). An artificial receptor based on MIPs was prepared by precipitation polymerization using phenylphosphonic acid (PPA) as a template, Ti4+-immobilized ethylene glycol methacrylate phosphate as functional monomer, poly(ethylene glycol) diacrylate and N,N'-methylenebisacrylamide as crosslinkers, and deionized water as a porogen. The performance of MIPs was first evaluated by rebinding PPA, and then a competition experiment was conducted to assess the selectivity and specificity for PPA when mixed with benzoic acid, a structural analog. The imprinting factor of the MIPs was up to 2.04, compared with just 0.24 for benzoic acid. The selectivity and capacity of the MIPs for pTyr peptides from a mixture of peptides were considerably higher than that of commercial TiO2. And after six rebinding cycles, there were still 95% adsorption efficiency retained. Finally, MIPs were used for pTyr enrichment from a complex sample containing pTyr peptide and tryptic digestion of β-casein, where it demonstrated a clear preference for the pTyr peptide over ones containing phosphorylated serine
Template and target information: phenylphosphonic acid, PPA, phosphotyrosine
Author keywords: Epitope imprinting, Molecularly imprinted polymers, Phenylphosphonic acid, precipitation polymerization, Tyrosine-phosphorylated peptides

  Template of Doom spoof movie poster mug  Mug featuring the name PeptideChemist spelled out in the single letter amino acid code  Chemists are fun customisable shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner