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Reference type: Journal
Authors: Nie GJ, Chen Z, Zheng ZM, Jin W, Gong GH, Wang L, Yue WJ
Article Title: Enhancement of transesterification-catalyzing capability of bio-imprinted tannase in organic solvents by cryogenic protection and immobilization.
Publication date: 2013
Journal: Journal of Molecular Catalysis B: Enzymatic
Volume: 94
Page numbers: 1-6.
DOI: 10.1016/j.molcatb.2013.04.007
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1381117713001069

Abstract: Improvement of transesterification-catalyzing capability of bio-imprinted tannase is a crucial question of whether to be efficiently utilized in organic media. As for biotransformation of tannic acid to propyl gallate, bio-imprinting technique can dramatically enhance the transesterification-catalyzing capability of tannase. In this work, both cryogenic protection and immobilization were utilized to further improve its apparent catalytic capability in organic media. The results show that Triton-X-100, mannose, and magnesium ion all have a positive effect on cryogenic protection of the tannase. Particularly, combinational application of the three cryoprotectants increases its catalytic performance by 2.7-fold factor. Also, immobilization further elevates its catalytic capability by 2.1 folds. Noteworthily, the coupling application of immobilization and cryo-protection can cause the conversion rate of substrate of the bio-imprinted tannase to increase to a promising 70%. Consequently, it will be helpful to fully utilize tannase in organic phase
Template and target information: bioimprinting, propyl gallate
Author keywords: Cryogenic protection, immobilization, bio-imprinting, Tannase, Propyl gallate, Tannic acid

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