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Reference type: Journal
Authors: Ratautaite V, Topkaya SN, Mikoliunaite L, Ozsoz M, Oztekin Y, Ramanaviciene A, Ramanavicius A
Article Title: Molecularly Imprinted Polypyrrole for DNA Determination.
Publication date: 2013
Journal: Electroanalysis
Volume: 25
Issue: (5)
Page numbers: 1169-1177.
DOI: 10.1002/elan.201300063

Abstract: In this study graphite electrodes modified by a thin DNA-imprinted polypyrrole layer, which was able to bind specific target-DNA, are reported. For this aim, electrochemical synthesis of polypyrrole was performed on a pencil graphite electrode by cyclic voltammetry (CV) or by potential pulse sequences (PPS). The modified electrode surface was used for electrochemical determination of target-DNA by differential pulse voltammetry. According to our best knowledge this is a first report on the application of DNA-imprinted polymer for the determination of target-DNA. The results showed that the molecularly imprinted polypyrrole (MIPPy) layer that formed on the carbon electrode surface was sensitive for target-DNA, while the nonimprinted polypyrrole layer was not sensitive to the same target-DNA. Comparison of electrodes modified using PPS and CV techniques is presented
Template and target information: DNA
Author keywords: molecularly imprinted polymers (MIPs), polypyrrole, DNA, electrochemical DNA biosensor, DNA-imprinted polymer

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