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Reference type: Journal
Authors: Huang J, Liu HQ, Men HF, Zhai YY, Xi QH, Zhang ZL, Zhang J, Yin ZZ, Li L
Article Title: Molecularly imprinted polymer coating with fluorescence on magnetic particle.
Publication date: 2013
Journal: Macromolecular Research
Volume: 21
Issue: (9)
Page numbers: 1021-1028.
DOI: 10.1007/s13233-013-1134-2

Abstract: In this research, molecular imprinting technology was employed to prepare magnetic, fluorescent molecularly imprinted polymer microspheres (fluorescent M-MIP) for recognition and separation of endocrine disrupting chemicals. The fluorescent M-MIP were prepared using Fe3O4@SiO2 magnetic nanoparticles combined with fluorescein (isothiocyanate) as fluorescent material with the surface molecularly imprinting method. The magnetic fluorescent molecularly imprinted polymers were characterized by fluorescence spectrophotometer, X-ray powder diffraction, vibration sample magnetic field meter, scanning and transmission electron microscopic methods. The results showed that the fluorescent M-MIP not only had excellent superparamagnetism and maintained the crystalline structure of the magnetic nanoparticles, but also stable fluorescence. The recognition selectivity of the magnetic fluorescence polymer was studied for template molecule and analogues. The results indicated that the fluorescent quenches of bisphenol A (the selective target) for fluorescent M-MIP were higher than that of the structural analogues, which illustrated the recognition selectivity for bisphenol A. Simultaneously, the fluorescent magnetic non-imprinted polymers (M-NIPs) had much higher fluorescent quenches than the fluorescent M-NIPs in the processes of rebinding. Therefore, the fluorescent M-MIP technology can be used for the recognition, magnetic separation and detection of bisphenol A by fluorescence spectrometry without any time-consuming elution
Template and target information: bisphenol A, BPA
Author keywords: magnetic fluorescent molecularly imprinted polymer (fluorescent M-MIP), fluorescein isothiocyanate, magnetic separation, fluorescence analysis

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