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Abstract: An alternative molecular imprinting technique is applied to the preparation of polymeric membranes for optical resolution. In this technique, the "molecular memory" of the print molecule is introduced in the membrane during the membrane preparation process. It was found that a membrane which contains an oligopeptide residue from a D-amino acid and is imprinted by D-isomer recognizes the D-isomer in preference to L-isomer, and vice versa. As for the membranes from a derivative of natural polymer or non-chiral synthetic polymers, the D-isomer imprinted polymeric membranes recognize D-isomer in preference to L-isomer, and vice versa. Enantioselective electrodialysis is attained with the present membranes reflecting their adsorption selectivity.