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Reference type: Journal
Authors: Zelnícková H, Rejtharová M
Article Title: Determination of 5-nitroimidazoles in various types of matrices using molecular imprinted polymer purification.
Publication date: 2013
Journal: Food Additives & Contaminants: Part A
Volume: 30
Issue: (6)
Page numbers: 1123-1127.
DOI: 10.1080/19440049.2013.780211

Abstract: Rapid and sensitive methods based on LC-MS/MS using positive electrospray ionisation have been developed for the determination of nine 5-nitroimidazoles and their three hydroxylated metabolites in blood serum, egg and muscle samples. The methods use a new type of column based on molecularly imprinted polymer for the cleanup of primary extracts of samples. A validation study was carried out according to criteria (accuracy, linearity, repeatability, reproducibility, ruggedness and specificity) and the requirements of Commission Decision 2002/657/EC. The methods with molecularly imprinted polymers are simple, fast and selective for the extraction of 5-nitroimidazoles from different matrices. They provide high recovery, good reproducibility, clean extracts and low background signals. The decision limits and detection capabilities were lower than the recommended minimum required performance limits in every matrix. These procedures could also be used as screening and confirmatory methods for the monitoring of veterinary drug residues
Template and target information: nitroimidazoles
Author keywords: nitroimidazoles, serum, Egg, muscle, molecularly imprinted polymer, LC-MS, MS

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