Abstract: Synthetization of nicotine imprinted polymer (MIP)-derivatized silica monolithic column was carried out based on in situ polymerization on the surface of macroporous silica gel-chemically modified as the matrix. Structural characterization was performed by FTIR,SEM,and the total pores and microspores volume were measured though size exclusion chromatography. In HPLC system,chromatographic condition was tested. Frontal analysis was utilized for producing absorption isotherm for the MIP monolithic column. Additionally,adsorption thermodynamic behavior of the MIP was studied. The result showed that selectivity factors of nicotine were 4.31 and 2.95 relative to 8-hydroxyquinoline and pyridine respectively,and the optimized chromatographic conditions was pH=7.5. Freundlich model was shown better than Langmuir model to describe isotherm adsorption behavior of MIP monolith toward nicotine with a heterogeneity index of 0.841. Thermodynamic analysis indicated a spontaneous exothermic process for the adsorption of template on the MIP monolithic column.
Template and target information: nicotine
Author keywords: molecular imprinted monolith, In situ polymerization, silica, nicotine, molecular recognition